Abstract

Objective: To study the use of red and green LASER in retinoblastoma seeds. Methods: Experimental study in enucleated RB eyeballs. Laser from a Class III Laser product. Complies with 21 CFR, Wavelength 532 nm were used in laboratory set up under the objective of compound microscope (Zeiss, Axioskop 40, Germany) without tagging in different RB seeds. Results: In six years study total RB cases seen in enucleated eyeballs were 59 and out of that, 35 cases had different RB seedings at different locations. RB seeds in anterior chamber (n-1 case), over crystalline lens (n=3 cases), vitreous (n=19 cases), retinal (n=1 case), RPE (n=2 cases), Sub-retinal (n=1 case), vitreous and sub-retinal (n=7 cases); calcified seeds were seen in (n=2 cases). Both the calcified seeds were seen in regressed RB in phthisical eyes. With red and green Laser, RB seeds had shown varied changes. Conclusion: Proper intensity of Laser can detect the RB seeds.