Abstract

Purpose:To demonstrate stepwise pixel density analysis for quantitative evaluation of aqueous humor outflow pathways(AHOP) by aqueous angiography(AA) using Image J software.Method and study design :Ten patients of primary open angle glaucoma who underwent AA, still images at 60 seconds were taken and analysed using ImageJ 1.46R Image Processing and Analysis Software. Two concentric circles, one at limbus and another 500 microns from it were drawn using tools from image J. After dividing them into 8 equal sectors the mean pixel density was calculated for each sector.Results:Mean pixel density in Nasal(N) Upper(U) and Lower(L) is 36.1 & 35.9 respectively Temporal(T) U & L 19.5 & 20 respectively, Superior N & T 22.7 & 18.5 respectively, Inferior N & T 20.2 & 12.1 respectively Conclusion: (N) quadrant & specifically UN quadrant had maximum number of AHOP and (T) quadrant, specifically IT quadrant had lesser number of AHOP.