FP2814 : Modulation of conjunctival lymphatics with retinoic acid and VEGF-C in rat model glaucoma surgery

  • Chief & Presenting Author : Dr.Faisal T. T.
  • Co Author : Prof. Surishti Raj, Prof.Sushmita Kaushik, Dr.Surinder S Pandav

Abstract

Design: Animal experiment study
Purpose:To study the effects of synthetic retinoic acid (EC23) and VEGF-C potential to induce lymphangiogenesis on rat model guarded filtering surgery (GFS)
Method: 5 Sprague-Dawley rats were allocated in 5groups (total 25) each were underwent GFS combined with either collagen implant, implant soaked with 10µg VEGFC/10nM EC23 or 10nM EC23 subconjunctival injection in right eye. Left eyes were used as control. Eyes where enucleated at day 14 and each specimen was stained with Masson trichome, CD-31 and D2-40
Results:Subconjunctival injection of EC23 (P=.005) or VEGF C (P=.001) application leads to significant increase in lymphatic channel density. The lymphatic density score was significantly (p=.007) higher in VEGF-C group. VEGF-C and EC23 had no significant effect on the blood vessel count and the amount of fibrosis.
Conclusion:Both EC32 and VEGF-C leads to lymphangiogenesis in rat model GFS and VEGF-C is found to be more potent lymphangiogenic agent.

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