Abstract

Design: Prospective comparative
Purpose: To assess aqueous humor(AH) cytokine profiles in primary open-angle glaucoma eyes and correlate with disease severity
Methods:Ethics Board approval obtained from 2 participating centers. Informed consents obtained from 3 groups (control, mild, and advanced glaucoma;n=10 each) based on visual field MD values. AH samples (100 μL per eye) were collected at the start of a scheduled surgery and subjected to a panel of 8000 cytokines.
Results:The advanced glaucoma group showed significantly higher levels of proinflammatory cytokines (IL-1, IL-17, IL-6), immune modulators (CD74, growth-related protein), angiogenic factors (Angiopoietin-1/2, endoglin), and structural proteins (matrix metalloproteinases, desmin). Gene ontology (GO) analysis revealed multiple pathways associated with immune activation in the advanced group.
Conclusion:Upregulation of inflammatory, immune, angiogenic and tissue remodeling pathways may contribute to glaucoma progression.

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