Abstract

Study Design:Prospective comparative
Purpose:To compare the lipid mediator profile in the aqueous humor (AH) of primary open-angle glaucoma (POAG) patients.
Methods:AH samples from 16 POAG patients and 18 controls (undergoing routine cataract surgery) were analyzed using LC-MS. Each eye provided 100 μL of AH, immediately snap-frozen at -80°C. Lipidomic analysis covered 40 polyunsaturated fatty acids and lipid mediators. Informed consent was obtained. The study was approved by Research and Ethics Boards of 2 participating institutions(#18-5419).
Results:Age and sex distributions were comparable for both groups. Significant differences were observed in arachidonic acid (1328.0±322.0 vs. 643.1±130.6, p=0.001), lipoxin A4 (0.79±0.14 vs. 0.32±0.10, p=0.01), and 12-hydroxyeicosapentaenoic acid (0.35±0.17 vs. undetected, p=0.04).
Conclusions:Glaucomatous eyes exhibit elevated levels of lipid mediators, suggesting a potential role for arachidonic acid metabolites in glaucoma pathogenesis.