Abstract

Vitreous biopsy and diagnostic vitrectomy is routinely done in many infective, inflammatory and masquerade conditions to arrive to the tissue specific diagnosis. Considering small available volume of the vitreous it is not uncommon to get insufficient samples. Moreover, diluted samples could decrease the yield of getting proper diagnosis.

In this video, we demonstrate two cases of suspected neoplastic conditions in the eye in which the entire vitreous biopsy and diagnostic vitrectomy was done under air. In this technique, 1 cc of undiluted vitreous fluid was obtained using cutter with active aspiration given by an assistant. After that instead of turning on saline through infusion, air was pumped through infusion and entire diagnostic vitrectomy was done under air. This helped in getting more (almost 3 cc) undiluted vitreous fluid.

This technique could be further validated doing a prospective study.